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RNAi

 

  • Full-sites siRNA Library Construction Kit
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  • Gene Target™ siRNA Set
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  • siRNA Plasmid Construction
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  • miRNA Plasmid Construction
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  • Real-Time PCR
  • Gene services

     

  • Gene Synthesis
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  • DNA Sequencing
  • Nucleic Acids Product

     

  • cDNA Library
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  • cDNA Clone
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  • Total RNA
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  • RISO™ RNA Isolation Reagent
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  • Agarose Gel DNA Extraction Kit
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  • Plasmid DNA Mini Purification Kit
  • Protein

     

  • Taq DNA Polymerase
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  • Pfu DNA Polymerase
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  • Protein Expression & Purification
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      Find the most effective siRNA target sequence for you!

    Order call now:+86 513 85175207 or email us:order@biomics.cn
     
       
         
     

    The siRNA products of Biomics are developed with our patented gene silencing technology, which consist of four siRNA clones with different sites and can eliminate off-target effect and enhance silencing efficiency. The four clones can be used in combination or separately. All the products have been confirmed with gene sequencing thus a 100% homology can be achieved.

    Our products have the following advantages over their peer products:

    No extra MCS (Multiple Cloning Site) sequences existing on the siRNA between the initiation signal "AAAAA" and the termination signal "TTTTT" of promoters (U6 and H1),thus silence reaction specificity can be increased;

    GFP of the pU6H1-GFP vector enables to monitor transfection efficiency when expression in cells;

    A total siRNA expression cassettes (U6-siRNA-H1) can be easily sub-cloned to other viral siRNA expression vectors (e.g., Retroviral, Adenoviral and Lentiviral vectors), thus can be widely used for each purpose;

    No secondary structure (such as hairpin) on the siRNA, thus be stable in above viral siRNA expression vectors.

    Expression Vector pU6H1-GFP
     
    Transfection result of pU6H1-GFP
    Vision under fluorescence microscopes 24 hours after Hela cells being transfected with siRNA expression vectors

    Picture 1 24 hours after Hela cells being transfected with siRNA expression vectors (×100)

    Picture 2 24 hours after Hela cells being transfected with siRNA expression vectors (×100)

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